Merck
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A9452

Sigma-Aldrich

Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Goat/Sheep IgG
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

conjugado

peroxidase conjugate

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

secondary antibodies

clon

GT-34, monoclonal

formulario

lyophilized powder

species reactivity

bovine, sheep, goat

should not react with

rat, chicken, mouse, horse, feline, pig, canine, guinea pig, rabbit, human

envase

vial of 0.5 mL

technique(s)

direct ELISA: 1:30,000
dot blot: 1:160,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

isotipo

IgG1

temp. de almacenamiento

2-8°C

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Descripción general

IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Aplicación

ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).

Acciones bioquímicas o fisiológicas

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy.

Forma física

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

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Certificado de origen

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