B9655

Sigma-Aldrich

Anti-Avidin antibody, Mouse monoclonal, biotin conjugate

clone WC19.10, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Avidin
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

WC19.10, monoclonal

formulario

buffered aqueous solution

aplicaciones

direct ELISA: suitable
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotipo

IgG1

conjugado

biotin conjugate

enviado en

dry ice

temp. de almacenamiento

−20°C

Descripción general

The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.
Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Inmunógeno

Purified egg white avidin.

Aplicación

Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women

Acciones bioquímicas o fisiológicas

Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays.

Forma física

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Chapter 16 - Biotechnological Approaches for Insect Pest Management
Integrated Pest Management Current Concepts and Ecological Perspective, 311-335 (2014)
Murine monoclonal anti-avidin antibodies enhance the sensitivity of avidin-biotin immunoassays and immunohistologic staining
Cassano WF, et al.
Journal of Immunological Methods, 117(2), 169-174 (1989)
Spontaneous and spindle poison-induced micronuclei and chromosome non-disjunction in cytokinesis-blocked lymphocytes from two age groups of women
Bakou K, et al.
Mutagenesis, 17(3), 233-239 (2002)
E O'Connor et al.
Journal of immunological methods, 229(1-2), 155-160 (1999-11-11)
The ability to detect a protein is always limited to the sensitivity of the assays available. Progress in improving the sensitivity of protein detection will allow a more complete understanding of biological systems. Of particular interest to the field of...

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