Merck
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C1805

Sigma-Aldrich

Anti-CD4 antibody, Mouse monoclonal

clone Q4120, purified from hybridoma cell culture

Synonym(s):
Anti-CD4 antibody, Mouse monoclonal
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

Q4120, monoclonal

formulario

buffered aqueous solution

mol peso

59 kDa

species reactivity

human

technique(s)

flow cytometry: 5 μL using 1 × 106 cells

isotipo

IgG1

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

2-8°C

Gene Information

human ... CD4(920)

Descripción general

Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. Cluster of differentiation 4 (CD4) human cell surface glycoprotein (59kDa) belongs to the immunoglobulin superfamily. It is expressed on the helper/inducer T cell subset, which is found on the majority of peripheral blood neutrophils, most cortical and mature medullary thymocytes, microglial cells, dendritic cells and on some malignancies of T cell origin.

Especificidad

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Inmunógeno

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Aplicación

Monoclonal Anti-CD4 antibody produced in mouse has been used in indirect immunofluorescent staining.

Acciones bioquímicas o fisiológicas

Cluster of differentiation 4 (CD4) molecule binds to the major histocompatibility complex (MHC) class II molecules during the interaction of CD4+ T cells with antigen presenting cells or with target cells. It also serves as a high affinity cellular receptor for the GP 120 envelope glycoprotein of the human immunodeficiency virus (HIV-1, HIV-2). The CD4 molecule is involved in the adhesion of T-lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins. Lower levels of CD4 have been detected in monocytes, macrophages and granulocytes.

Descripción de destino

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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