Origen línea celular
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Descripción línea celular
The antibody is directed against the envelope protein of the wild type and vaccine strains of yellow fever virus. It does not cross react with other flaviviruses.
Yellow fever virus, strain 17D
Medio de cultivo
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS)
Rutina de subcultivo
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
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