E6775

Sigma-Aldrich

Anti-EphA3 antibody produced in goat

affinity isolated antibody, lyophilized powder

Número MDL:

origen biológico

goat

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

polyclonal

formulario

lyophilized powder

species reactivity

mouse

aplicaciones

indirect ELISA: 0.5-1.0 μg/mL
western blot: 0.1-0.2 μg/mL

conjugado

unconjugated

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

mouse ... Epha3(13837)

Descripción general

Eph family of tyrosine kinase receptors mediates cell-cell attachment and aggregation by binding to specific ligands, ephrins. The Eph family consists of 14 structurally related receptors that are either membrane linked or are transmembrane with overlapping affinity for ligands. The transmembrane receptors EphA and EphB (ELK) subclasses signal via distinct mediators in the cytoplasm. EphA3 bind ephrin A and regulates the signals for neural crest formation and the migration of neurons during vascular cell assembly, development and angiogenesis . Anti-EphBA3 antibody recognizes mouse EphA3. The antibody shows approximately 10% cross reactivity with recombinant mouse EphA6 and recombinant mouse EphA7 and 5% cross reactivity with recombinant human EphA1, recombinant mouse EphA2, recombinant mouse EphA4, recombinant mouse EphA8, and recombinant rat EphA5.

Especificidad

By immunoblotting and ELISA, the antibody shows ~10% cross-reactivity with recombinant mouse EphA6, mouse EphA7 and 5% cross-reactivity human EphA1, mouse EphA2, mouse EphA4, mouse EphA8, and rat EphA5.

Inmunógeno

recombinant mouse EphA3, extracellular domain, expressed in NSO cells.

Aplicación

Anti-EphA3 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, the recommended concentration is 0.5-1.0 μg/ml.

Forma física

Lyophilized from 0.2 μm filtered phosphate buffered saline solution.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
D Orioli et al.
Trends in genetics : TIG, 13(9), 354-359 (1997-09-01)
Eph receptors and their ligands have been implicated in axonal pathfinding during the formation of neuronal networks. Whereas other guiding molecules, such as netrins and most semaphorins, are diffusible factors that can guide axons over considerable distances, Eph receptors and...
H U Wang et al.
Neuron, 18(3), 383-396 (1997-03-01)
In vertebrate embryos, neural crest cell migration and motor axon outgrowth are restricted to rostral somite halves by repulsive factors located in the caudal somite compartment. We show that two Eph family transmembrane ligands, Lerk2 and HtkL, are expressed in...
E Stein et al.
Genes & development, 12(5), 667-678 (1998-04-16)
Eph family receptor tyrosine kinases (including EphA3, EphB4) direct pathfinding of neurons within migratory fields of cells expressing gradients of their membrane-bound ligands. Others (EphB1 and EphA2) direct vascular network assembly, affecting endothelial migration, capillary morphogenesis, and angiogenesis. To explore...

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