E7403

Sigma-Aldrich

Anti-S1P1, C-Terminal antibody produced in rabbit

~1 mg/mL, fractionated antiserum, buffered aqueous solution

Sinónimos:
Anti-Endothelial Cell Differentiation Gene-1, Anti-EDG-1
Número MDL:
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

200

forma del anticuerpo

fractionated antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~44 kDa by SDS-PAGE

species reactivity

human

concentración

~1 mg/mL

aplicaciones

western blot: suitable

conjugado

unconjugated

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... S1PR1(1901)

Descripción general

Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein encoded by the S1PR1 gene in humans. It is a bioactive sphingolipid present at high concentrations in plasma and lymphatic fluid. S1P plays a critical role in regulating lymphocyte trafficking mainly through sphingosine-1-phosphate receptor 1 (S1PR1). It is a blood-borne lysosphingolipid that acts to promote endothelial cell (EC) barrier function. In plasma, it is associated with both high density lipoproteins (HDL) and albumin.

Inmunógeno

synthetic peptide (approximately 2.5 kDa) derived from the C-terminal region of the EDG-1/S1P1 receptor.

Aplicación

Anti-S1P1, C-Terminal antibody produced in rabbit is suitable for western blotting at a working concentration of 5 to 10 μg/ml using RH7777 cells transfected with full-length EDG-1 receptor.

Acciones bioquímicas o fisiológicas

Sphingosine-1-phosphate (SPP) activates the heterotrimeric guanine nucleotide binding protein (G protein)-coupled orphan receptor EDG-1. EDG-1 is a high-affinity receptor for the bioactive lipid mediator SPP. It binds to SPP with high affinity and specificity. Overexpression of EDG-1 induces exaggerated cell-cell aggregation, enhances expression of cadherins and formation of well-developed adherens junctions. It is essential for lymphocyte egress from secondary lymphoid organs and acts as a validated drug target for the treatment of autoimmune diseases. Activated EDG-1 induces an immediate increase in intracellular calcium and is able to increase S1P1 phosphorylation.

Forma física

Supplied in phosphate buffered saline, pH 7.3, containing 0.08% sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Alemania

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Development of a selective S1P1 receptor agonist, Syl930, as a potential therapeutic agent for autoimmune encephalitis.
Jin J, Hu J, Zhou W, et al.
Biochemical Pharmacology, 90(1), 50-61 (2014)
C H Liu et al.
Molecular biology of the cell, 10(4), 1179-1190 (1999-04-10)
The endothelial-derived G-protein-coupled receptor EDG-1 is a high-affinity receptor for the bioactive lipid mediator sphingosine-1-phosphate (SPP). In the present study, we constructed the EDG-1-green fluorescent protein (GFP) chimera to examine the dynamics and subcellular localization of SPP-EDG-1 interaction. SPP binds...
Marco Antonio Morquecho-León et al.
Biochimica et biophysica acta, 1843(2), 327-334 (2013-11-19)
The role of protein kinase C (PKC) isozymes in phorbol myristate acetate (PMA)-induced sphingosine 1-phosphate (S1P) receptor 1 (S1P1) phosphorylation was studied. Activation of S1P1 receptors induced an immediate increase in intracellular calcium, which was blocked by preincubation with PMA....
M J Lee et al.
Science (New York, N.Y.), 279(5356), 1552-1555 (1998-03-21)
The sphingolipid metabolite sphingosine-1-phosphate (SPP) has been implicated as a second messenger in cell proliferation and survival. However, many of its biological effects are due to binding to unidentified receptors on the cell surface. SPP activated the heterotrimeric guanine nucleotide...
Michael J Kluk et al.
Laboratory investigation; a journal of technical methods and pathology, 93(4), 462-471 (2013-02-20)
Classical Hodgkin lymphoma (CHL), a neoplasm of abnormal B lymphocytes (Hodgkin-Reed-Sternberg (HRS) cells), has been described to have a typical pattern of clinical presentation and dissemination often involving functionally contiguous lymph nodes. Despite the progress made in understanding CHL pathophysiology...

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