EMS0006

Trypsin Protease

Hydrolyzes peptide bonds at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris

• Sinónimos: Mass Spectrometry Trypsin, Proteomics grade Trypsin, rTrypsin
• Código UNSPSC: 12352204
• NACRES: NA.26

Propiedades

• Nombre del producto: Recombinant Trypsin, Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris
• recombinante: expressed in Pichia pastoris
• Nivel de calidad: 200
• grado: Proteomics Grade
• Formulario: lyophilized powder
• actividad específica: ≥10,000 units/mg protein
• idoneidad: suitable for ; suitable for mass spectrometry
• Nº de acceso UniProt: P00761
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Trypsin is a major proteolytic enzyme, synthesized as a preproenzyme by pancreas and is stored as proenzyme trypsinogen in secretory granules.[1] Trypsin belongs to the family of serine proteases that are characterized by the catalytic triad His57, Asp102 and Ser195.[2] Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.[3] The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0. This product is prepared from recombinant trypsin, porcine sequence. It is naturally devoid of chymotryptic activity. This high-quality trypsin is suitable for proteomics use.

Acciones bioquímicas o fisiológicas

Trypsin plays an important role in the digestion of consumed protein and contributes to the activation of other proteolytic enzymes like chemotrypsin and elastase.[1]

Información de seguridad

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H290,H314,H317,H334,H335,H411
• Consejos de prudencia: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Clasificaciones de peligro: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• Órganos de actuación: Respiratory system
• Riesgos supl.: EUH071
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable