Documentos
G1044
Monoclonal Anti-Granzyme B antibody produced in mouse
clone GrB7, tissue culture supernatant
Número MDL:
NACRES:
NA.41
Nivel de calidad
200
origen biológico
mouse
forma del anticuerpo
tissue culture supernatant
antibody product type
primary antibodies
clon
GrB7, monoclonal
descripción
not suitable for immunocytochemistry (frozen sections)
mol peso
antigen 33 kDa
species reactivity
human
aplicaciones
immunocytochemistry: 1:20 using pretreated tissues
western blot: suitable
conjugado
unconjugated
Nº de acceso UniProt
enviado en
dry ice
temp. de almacenamiento
−20°C
Gene Information
human ... GZMB(3002)
Especificidad
Does not cross-react with granzyme A.
Inmunógeno
recombinant human granzyme B.
Aplicación
Monoclonal Anti-Granzyme B antibody is suitable for western blot and immunocytochemistry at a dilution of 1:20 using pretreated tissues.
Acciones bioquímicas o fisiológicas
Granzyme B, neutral serine protease, is expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. It contains a leader sequence (cleaved by a signal peptidase) and two amino acid prodomains (cleaved by the lysosomal cysteine protease DPPI). It plays a major role in the caspase dependent apoptotic pathway by activating most of the caspases in vitro and in vivo. It cleaves the aspartic acid residues, to facilitate cell death by various pathways. It has been shown that granzyme B has capacity to facilitate cytochrome C release from the mitochondria in a caspase independent way.
Forma física
Supplied in serum-free culture medium containing 1% bovine serum albumin and 0.1% sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK Alemania
2
Certificado de Análisis
Certificado de origen
Más documentos
S Shresta et al.
Current opinion in immunology, 10(5), 581-587 (1998-10-31)
CD8+ cytotoxic lymphocytes, natural killer cells and lymphokine-activated killer cells depend primarily on the perforin/granzyme system to kill their targets, while CD4+ T cells utilize Fas and other mechanisms to induce cell death. The molecular mechanisms used by these pathways...
J A Heibein et al.
Journal of immunology (Baltimore, Md. : 1950), 163(9), 4683-4693 (1999-10-21)
CTLs kill targets by inducing them to die through apoptosis. A number of morphological and biochemical events are now recognized as characteristic features of the apoptotic program. Among these, the disruption of the inner mitochondrial transmembrane potential (Delta Psi m)...
J A Kummer et al.
Journal of immunological methods, 163(1), 77-83 (1993-07-06)
The human serine proteases granzymes A and B are expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise specific...
J J Oudejans et al.
Blood, 87(9), 3844-3851 (1996-05-01)
To get insight into the failure of the immune system to eradicate Epstein-Barr virus (EBV) harboring Hodgkin and Reed-Sternberg cells (H-RS cells), expressing the latent membrane protein 1 (LMP1), we analyzed major histocompatibility complex (MHC) class I expression on H-RS...
P C de Bruin et al.
Blood, 84(11), 3785-3791 (1994-12-01)
T-cell non-Hodgkin's lymphomas can be considered the neoplastic equivalents of immunologically functional, site-restricted T lymphocytes. Little is known about the occurrence and clinical behavior of T-cell lymphomas that are the neoplastic equivalents of different functional T-cell subsets. Here, we investigated...
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnicoAsistencia técnica
Servicio de atención al clienteContacte con nosotrosPreguntas frecuentesFichas técnicas de seguridad (SDS)Certificados (COA y COO)Calidad y registrosCalculadoras y aplicacionesSeminarios virtualesRedes sociales
Merck
Investigación. Desarrollo. Producción.
Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos
Sigma-Aldrich®Soluciones BioReliance®Soluciones Millipore®Soluciones SAFC®Soluciones
Milli-Q®Soluciones Supelco®
© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.
Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.