Merck
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G3272

Sigma-Aldrich

Guanidine hydrochloride

for molecular biology, ≥99%

Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.31

origen biológico

synthetic (organic)

Nivel de calidad

400

grado

for molecular biology

ensayo

≥99%

formulario

crystalline powder

technique(s)

RNA extraction: suitable

impurezas

≤0.3% water (Karl Fischer)

color

white

pH

(25 °C, 4.6 - 6/573 g/L)

mp

180-185 °C (lit.)

solubilidad

H2O: 6 M, clear, colorless

densidad

1.3 g/cm3 (lit.)

trazas de catión

Pb: ≤5 ppm

Absorción UV

λ: 260 nm Amax: ≤0.10
λ: 290 nm Amax: ≤0.05

actividad extraña

DNase, RNase, none detected

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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Aplicación

Guanidine hydrochloride has been used:
  • as a component of the extraction buffer for the extraction of proteoglycans[1]
  • in extraction during protein fractionation of ATDC5 cell lines[2]
  • as a chemical additive to study its effective absorbance spectra in structural analysis[3]
  • to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer[4]
  • as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study[5]
  • in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Envase

100, 500 g in poly bottle
1, 2 kg in poly bottle

Acciones bioquímicas o fisiológicas

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule.[6][7] It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo.[6] It is a potent denaturant and inactivator of several enzymes and proteins.[7][8] GuHCl can inactivate aminoacylase and papain.[8] It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor.[8] At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).[9]

Precaución

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Nota de preparación

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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