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G4505

Sigma-Aldrich

Guanidine hydrochloride

≥99% (titration), organic base and chaeotropic agent

Synonym(s):
Guanidium Chloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
Número de EC:
Número MDL:
ID de la sustancia en PubChem:

origen biológico

synthetic (organic)

Nivel de calidad

400

ensayo

≥99% (titration)

formulario

crystalline powder

technique(s)

RNA extraction: suitable

impurezas

≤0.3% water (Karl Fischer)

color

white

pH

(25 °C, 4.6 - 6 at 573 g/L)

mp

180-185 °C (lit.)

solubilidad

H2O: 6 M

densidad

1.3 g/cm3 (lit.)

absorción

≤0.10 at 260 nm in H2O at 6 M

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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Aplicación

Guanidine hydrochloride (GdnHCl) has been used:
  • to perform absolute quantitation of amyloid-beta (Aß) isoforms in plasma[1]
  • in protein carbonylation assay[2]
  • for the quantification of glycosaminoglycans (GAG)[3]
  • for denaturation of cow′s milk allergens[4]
  • as a control in antiviral screening tests with coxsackievirus B3 (CVB3)[5]
  • in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Envase

25, 100, 500 g in poly bottle
1 kg in poly bottle
2, 10, 25 kg in poly drum
G4505-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Acciones bioquímicas o fisiológicas

Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule.[6][7] It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity.[8] GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo.[7] It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase.[8] GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).[9]

Precaución

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri free-flowing product number RDD001.

Nota de preparación

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Información legal

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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