G5421

Sigma-Aldrich

Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat

clone 67604, purified immunoglobulin, lyophilized powder

Sinónimos:
Anti-MGI-IG, Anti-Csf3, Anti-G-CSF, Anti-Csfg
NACRES:
NA.41

Nivel de calidad

100

origen biológico

rat

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

67604, monoclonal

formulario

lyophilized powder

species reactivity

mouse

aplicaciones

capture ELISA: 2-8 μg/mL
neutralization: suitable
western blot: 1-2 μg/mL

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

mouse ... Csf3(12985)

Descripción general

Mature myeloid cells are derived from bone-marrow derived precursor cells by the activity of three colony stimulating factors, granulocyte/macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF) and granulocyte colony-stimulating factor (G-CSF). The expression of G-CSF is the result of activation of pathways in response to tumor necrosis factor-α, LPS and toll-like receptor ligands. The pathways that induce the effects of G-CSF are PI3K, ERK1/2 and p38. The generation of neutrophils and induction of the circulation of hematopoietic stem cells from the bone marrow are the main functions of G-CSF. There are additional activities attributed to G-CSF such as neuroprotection, immunomodulation and generation of cardiac cells and dendritic cells. Large quantities of G-CSF are secreted in a variety of myeloid and non-myeloid cancers and rheumatoid arthritis
Monoclonal anti-granulocyte colony stimulating factor recognizes mouse G-CSF. The antibody shows less than 0.06% cross-reactivity with recombinant human G-CSF.

Inmunógeno

purified, recombinant mouse granulocyte colony stimulating factor, expressed in E. coli.

Aplicación

Anti-granulocyte colony stimulating factor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA the recommended concentration range is 2-8 μg/ml. The antibody is also suitable for neutralization assays.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Lorraine Pelosof et al.
Epigenetics, 12(7), 540-550 (2016-12-06)
Epigenetic control of gene expression is a major determinant of tumor phenotype and has been found to influence sensitivity to individual chemotherapeutic agents. Glutathione peroxidase 3 (GPX3, plasma glutathione peroxidase) is a key component of cellular antioxidant regulation and its...
F Liu et al.
Immunity, 5(5), 491-501 (1996-11-01)
We have generated mice carrying a homozygous null mutation in the granulocyte colony-stimulating factor receptor (G-CSFR) gene. G-CSFR-deficient mice have decreased numbers of phenotypically normal circulating neutrophils. Hematopoietic progenitors are decreased in the bone marrow, and the expansion and terminal...
R R Ji et al.
Nature neuroscience, 2(12), 1114-1119 (1999-11-26)
We investigated the involvement of extracellular signal-regulated protein kinases (ERK) within spinal neurons in producing pain hypersensitivity. Within a minute of an intense noxious peripheral or C-fiber electrical stimulus, many phosphoERK-positive neurons were observed, most predominantly in lamina I and...
Sophie Pezet et al.
The European journal of neuroscience, 21(7), 1785-1797 (2005-05-05)
The serine/threonine kinase Akt/PKB has been implicated in cell survival signalling in many cell types, including the dorsal root ganglion (DRG). However, little is known about its role in physiological and pathophysiological conditions in the adult sensory and nociceptive system....
Sebastian Stösser et al.
Journal of molecular medicine (Berlin, Germany), 89(4), 321-329 (2010-11-17)
A variety of cancers are accompanied by debilitating pain, which constitutes the primary reason for poor quality of life in cancer patients. There is an urgent demand for the development of specific mechanism-based therapies against cancer pain. Recently, important advances...

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