G7765

Sigma-Aldrich

Gelatin from cold water fish skin

40-50% in H2O

Sinónimos:
Teleostean gelatin, Gelatine
Número de CAS:
Número MDL:
NACRES:
NA.61

Nivel de calidad

200

origen biológico

fish skin

formulario

liquid

mol peso

~60 kDa

contiene

0.15% propyl p-hydroxybenzoate as preservative
0.2% methyl p-hydroxybenzoate as preservative

concentración

40-50% in H2O

aplicaciones

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

temp. de almacenamiento

2-8°C

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Descripción general

Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.

Aplicación

Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.
Used as a blocking agent in immunochemistry.

Envase

250 mL in poly bottle
1 L in poly bottle

Acciones bioquímicas o fisiológicas

Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.

storage_class_code

12 - Non Combustible Liquids

WGK Alemania

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de Análisis

Certificado de origen

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product G7765, Gelatin from cold water fish skin, soluble in?      

    Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.

  4. What is the solution stability of Product G7765, Gelatin from cold water fish skin?

    The specification for shelf life is 2 years for unopened bottles stored at 2-8°C.  Each lot is assigned a retest date; this date is given on the Certificate of Analysis avialable from our website.

  5. What is the difference between type A and type B gelatin?

    Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2. 

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is the gel point of Product G7765, Gelatin from cold water fish skin?

    The gel point of Product G7765, Gelatin from cold water fish skin is 5-10°C. The product will be a gel when storing the product at 2-8°C.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress
Wehner K A, et al.
Molecular and Cellular Biology, 30(8), 2006-2016 (2010)
Synthesis and characterization of photocrosslinkable hydrogels from bovine skin gelatin
Suvarnapathaki S, et al.
Royal Society of Chemistry Advances, 9(23), 13016-13025 (2019)
Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues.
Freistadt M S, et al.
Molecular and Cellular Biology, 10(11), 5700-5706 (1990)
Soybean protein concentrate as a protein source for turbot Scophthalmus maximus L
O.J. Day and H.G. Plascencia GonzAlez
Aquaculture (Amsterdam, Netherlands), 4, 221-228 (2000)
Nobuo Terada et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 57(9), 883-888 (2009-05-28)
The purpose of this study was to clarify a previously controversial issue concerning glutamate (Glu) immunoreactivity (IR) in the inner segment (IS) of photoreceptors by using in vivo cryotechnique (IVCT) followed by freeze substitution (FS), which enabled us to analyze...

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