G8898

Sigma-Aldrich

Glycine

suitable for electrophoresis, ≥99%

Sinónimos:
Aminoacetic acid, Aminoethanoic acid, Glycocoll
Fórmula lineal:
NH2CH2COOH
Número de CAS:
Peso molecular:
75.07
Beilstein/REAXYS Number:
635782
Número de EC:
Número MDL:
eCl@ss:
32160406
ID de la sustancia en PubChem:

Nivel de calidad

200

ensayo

≥99%

formulario

powder

aplicaciones

electrophoresis: suitable
western blot: suitable

impurezas

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubilidad

H2O: 200 mg/mL, clear, colorless to faintly yellow

trazas de anión

chloride (Cl-): ≤70 ppm

trazas de catión

heavy metals (as Pb): ≤20 ppm

absorción

≤0.15 at 280 at 1 M

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

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Descripción general

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Aplicación

  • Glycine has been used for the preparation of gelatin composites scaffold.
  • It has been used in the elution buffer to extract protein using HiTrap protein G column.
  • It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Envase

500 g in poly bottle
1 kg in poly bottle

Acciones bioquímicas o fisiológicas

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Nota de análisis

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

storage_class_code

11 - Combustible Solids

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

Certificado de origen

The role of muscle-derived stem cells in bone tissue engineering.
Sun JS et al.
Biomaterials, 26, 3953-3953 (2005)
Quantitative immunofluorescent blotting of the multidrug resistance-associated protein 2 (MRP2).
Gerk PM
Journal of Pharmacological and Toxicological Methods, 63, 279-279 (2011)
Hames, B.D. and Rickwood, D.
Gel Electrophoresis of Proteins: A Practical Approach, 32-35 (1990)
Westermeier R
Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations (2016)
The newly developed monoclonal antibody SA7D6 exhibits potential for detection of Staphylococcus aureus.
Senevirathne A and Kim K-P
Food Science and Biotechnology, 24, 1177-1184 (2015)

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