Merck
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G9277

Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.31

origen biológico

synthetic (organic)

Nivel de calidad

200

grado

for molecular biology

ensayo

≥99%

formulario

powder or crystals

technique(s)

RNA extraction: suitable

color

colorless to white,

mp

120-122 °C (lit.)

solubilidad

water: 100 mg/mL, clear, colorless

trazas de catión

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

application(s)

agriculture

actividad extraña

DNase, RNase, none detected

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Aplicación

Guanidine thiocyanate has been used:
  • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation[1]
  • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay[2]
  • for DNA extraction from cerebrospinal fluid (CSF)[3]

Chaotropic agent and strong denaturant; solubilizes cells.

Envase

100, 250, 500 g in poly bottle

Acciones bioquímicas o fisiológicas

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples.[4] Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies.[5] It is used in combination with phenol−chloroform in RNA extraction.[6]

pictogramas

CorrosionExclamation mark

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Riesgos supl.

Código de clase de almacenamiento

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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