Merck
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H4034

Sigma-Aldrich

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número de CAS:
Peso molecular:
238.30
Beilstein:
883043
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

grado

BioPerformance Certified

Nivel de calidad

400

ensayo

≥99.5% (titration)

technique(s)

cell culture | mammalian: suitable

impurezas

endotoxin and total aerobic microbial count, tested

useful pH range

6.8-8.2

pKa (25 °C)

7.5

trazas de catión

Fe: ≤5 ppm

absorción

≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M

application(s)

diagnostic assay manufacturing

actividad extraña

DNase, RNase, NICKase, protease, none detected

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Aplicación

HEPES has been used:
  • To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
  • As a component of platelet suspension buffer
  • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
  • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
  • For the adjustment and maintenance of pH of biological solutions
  • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
  • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
  • As a component of keratinocyte and fibroblast culture medium

Envase

25 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Otras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Three-Dimensional Human Tissue Models of Wounded Skin
Egles C, et al.
Methods in Molecular Biology, 585, 345-359 (2010)
Blood compatibility of surfaces with superlow protein adsorption.
Zhang Z, et al.
Biomaterials, 29(32), 4285-4291 (2008)
Use of a new buffer in the culture of animal cells.
Williamson JD and Cox P
The Journal of General Virology, 2, 309-309 (1968)
Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.
Zimmermann J, et al.
Biophysical Journal, 102(2), 287-295 (2012)
Complex thermorheology of living cells.
Schmidt BUS, et al.
New Journal of Physics, 17(7), 073010-073010 (2015)

Protocolos

Protein Interactions

This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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