Merck
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I4131

Sigma-Aldrich

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Sinónimos:
Rat IgG
Número MDL:
NACRES:
NA.46

Nivel de calidad

300

conjugado

unconjugated

grado

reagent grade

ensayo

≥95% (SDS-PAGE)

formulario

essentially salt-free, lyophilized powder

temp. de almacenamiento

2-8°C

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Descripción general

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids, Immunoglobins have two heavy (H) and two light (L) chains, linked by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Rat IgG is purified from pooled normal rat serum by fractionation and ion-exchange chromatography.

Aplicación

IgG from rat serum has been used:
  • in immunolabeling
  • in flow cytometry
  • in enzyme linked immunosorbent assay
  • as a control in neutrophil depletion assay
Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG.

Envase

10, 50, 100 mg in poly bottle

Acciones bioquímicas o fisiológicas

IgG antibody have enormous therapeutic potential and its fragment crystallizable (Fc) region contributes for the development of therapeutic antibody.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

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Certificado de origen

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Product Information Sheet

Quotes and Ordering

Effect of interleukin-6 receptor blockage on renal injury in apolipoprotein E-deficient mice
Tomiyama-Hanayama M et al
American Journal of Physiology: Renal Physiology, 297, F679-F684 (2009)
Catharina D Commerford et al.
Cell reports, 25(13), 3554-3563 (2018-12-28)
Enlargement of the lymphatic vascular network in tumor-draining lymph nodes (LNs) often precedes LN metastasis, likely providing a lymphovascular niche for tumor cells. We investigated morphological and molecular changes associated with the lymphatic remodeling process, using the 4T1 breast cancer
D Hildeman et al.
Journal of virology, 71(12), 9672-9678 (1997-11-26)
Lymphocytic choriomeningitis virus (LCMV) infection of normal mice results in a fatal immunopathologic meningitis mediated by CD8+ cytotoxic T lymphocytes (CTL). We have previously shown that female beta2-microglobulin-deficient (beta2m-/-) mice, which are also deficient in CD8+ T cells, are susceptible
Neutrophils instruct homeostatic and pathological states in naive tissues
Casanova-Acebes M, et al.
The Journal of Experimental Medicine, 215(11), 2778-2795 (2018)
Transcription and methylation analyses of preleukemic promyelocytes indicate a dual role for PML/RARA in leukemia initiation
Gaillard C, et al.
Haematologica, 100(8), 1064-1075 (2015)

Artículos

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Protocolos

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Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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