I5029

Sigma-Aldrich

IgG1, Lambda from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Sinónimos:
Human IgG1-λ
Número MDL:
NACRES:
NA.46

Nivel de calidad

200

origen biológico

human

forma del anticuerpo

purified immunoglobulin

ensayo

>95% (SDS-PAGE)

conjugado

unconjugated

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, λ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4.Elevation in antigen specific IgG1 is associated with symptomatic infection and Th2-regulation. IgG1 λ from human myeloma can be used in quantitative ELISA.Human IgG1 λ react specifically with human IgG.

Aplicación

IgG1 λ from human myeloma can be used as reference antigen, blocking agent, immunoglobulin calibrator or coating protein in various immunoassay like ELISA, dot blot immunobinding, immunodiffusion, immunoelectrophoresis, hemagglutination, cell-binding assays and western immunoblotting.
The purified IgG1, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, λ was used as non-specific control in quantitative ELISA and solid-phase ELISA. A range of concentrations of IgG1, λ from 10 to 100 ng was used for quantitative ELISA. Human myeloma IgG1, λ was also used as control in size-exclusion chromatography to analyse purity of recombinant antibodies.

Forma física

Solution in 0.02 M Tris buffered saline, pH 8.0.

Nota de análisis

Identity is determined by immunoelectrophoresis and indirect ELISA.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

M J Day
Veterinary parasitology, 147(1-2), 2-8 (2007-05-01)
Infection with Leishmania may have different outcomes in genetically distinct individuals and the course of infection is determined by the nature of the host innate and adaptive immune response. Thus in experimentally infected mice, and in naturally infected dogs or...
Julie Jacobs et al.
Oncotarget, 8(43), 74058-74067 (2017-11-02)
In contrast to the negligible expression of the immunomodulating protein CD70 in normal tissue, we have demonstrated constitutive overexpression of CD70 on tumor cells in a subset of primary non-small cell lung cancer (NSCLC) biopsies. This can be exploited by...
Cristina Capodicasa et al.
Biotechnology and bioengineering, 114(12), 2729-2738 (2017-08-24)
Immunoglobulins A (IgA) are crucially involved in protection of human mucosal surfaces from microbial pathogens. In this work, we devised and expressed in plants recombinant chimeric antifungal antibodies (Abs) of isotype A (IgA1, IgA2, and scFvFcA1), derived from a murine...
Ken Ishii et al.
The Journal of investigative dermatology, 140(10), 1919-1926 (2020-03-07)
Anti-desmoglein (Dsg) 1 and Dsg3 IgG autoantibodies in pemphigus foliaceus and pemphigus vulgaris cause blisters through loss of desmosomal adhesion. It is controversial whether blister formation is due to direct inhibition of Dsg, intracellular signaling events causing desmosome destabilization, or...
A M Lew
Journal of immunological methods, 72(1), 171-176 (1984-08-03)
The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.

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