I6527

Sigma-Aldrich

Anti-Interleukin-4 Soluble Receptor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Sinónimos:
Anti-IL-4 sR
Número MDL:
NACRES:
NA.41

Nivel de calidad

100

origen biológico

goat

forma del anticuerpo

IgG fraction of antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

lyophilized powder

species reactivity

human

aplicaciones

indirect ELISA: suitable
neutralization: suitable
western blot: suitable

conjugado

unconjugated

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

human ... IL4R(3566)

Descripción general

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signalling stimulated by IL-4R leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4R signaling have been reported in cancers of breast, liver and renal cells
Anti-Interleukin-4 soluble receptor specifically recognizes human cell surface IL-4R.

Especificidad

The antibody will neutralize human cell surface IL-4 receptor mediated-bioactivity.

Inmunógeno

recombinant human IL-4 soluble receptor (IL-4 sR), expressed in Sf 21 insect cells.

Aplicación

Anti-Interleukin-4 soluble receptor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody is suitable for neutralization reactions at a working concentration of 5-10 μg/ml. This antibody was used for immunostaining of parasympathetic neurons from guinea pig and human neuroblastoma cells at a dilution of 1:200.

Forma física

Lyophilized from 51.5 μL of a 0.2μm filitered solution in PBS (pH7.4) with 5% trehalose.

Nota de preparación

Purified using Protein G.

storage_class_code

10 - Combustible liquids

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

Allison D Fryer et al.
The Journal of clinical investigation, 116(1), 228-236 (2005-12-24)
Eosinophils cluster around airway nerves in patients with fatal asthma and in antigen-challenged animals. Activated eosinophils release major basic protein, which blocks inhibitory M2 muscarinic receptors (M2Rs) on nerves, increasing acetylcholine release and potentiating vagally mediated bronchoconstriction. We tested whether...
M Stein et al.
The Journal of experimental medicine, 176(1), 287-292 (1992-07-01)
Expression of the macrophage mannose receptor is inhibited by interferon gamma (IFN-gamma), a T helper type 1 (Th-1)-derived lymphokine. Interleukin 4 (IL-4), a Th-2 lymphocyte product, upregulates major histocompatibility class II antigen expression but inhibits inflammatory cytokine production by macrophages....
E M Coccia et al.
International immunology, 12(7), 977-985 (2000-07-06)
NO is a labile radical involved in several immunological, antimicrobial and inflammatory processes. In macrophages, NO formation is catalyzed by the cytokine-inducible enzyme inducible NO synthase (iNOS). The importance of IFN regulatory factor (IRF)-1 and of the signal transducers and...
Alberto Mantovani et al.
Trends in immunology, 25(12), 677-686 (2004-11-09)
Plasticity and functional polarization are hallmarks of the mononuclear phagocyte system. Here we review emerging key properties of different forms of macrophage activation and polarization (M1, M2a, M2b, M2c), which represent extremes of a continuum. In particular, recent evidence suggests...
Jane Gilmour et al.
Immunology, 124(4), 437-444 (2008-05-16)
The mechanism of differentiation of naïve T cells to a variety of effector lineages, but particularly to T helper type 1 (Th1) and Th2 cells, has been the subject of intense scrutiny over the past two decades. Studies have revealed...

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