I9516

Sigma-Aldrich

2-Propanol

BioReagent, for molecular biology, ≥99.5%

Sinónimos:
IPA, Isopropanol, sec-Propyl alcohol, Isopropyl alcohol
Fórmula lineal:
(CH3)2CHOH
Número de CAS:
Peso molecular:
60.10
Beilstein/REAXYS Number:
635639
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.52

Nivel de calidad

200

grado

for molecular biology

densidad de vapor

2.1 (vs air)

presión de vapor

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

línea de producto

BioReagent

ensayo

≥99.5%

formulario

liquid

temp. de autoignición

750 °F

lim. expl.

2.0-12.7 %, 93 °C

aplicaciones

DNA extraction: suitable
RNA extraction: suitable

impurezas

≤0.2% water
water

color

colorless

índice de refracción

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubilidad

water: soluble (completely)

densidad

0.785 g/mL at 25 °C (lit.)

idoneidad

suitable for precipitation of nucleic acids

temp. de almacenamiento

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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Descripción general

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Aplicación

2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Envase

25, 500 mL in glass bottle

pictogramas

FlameExclamation mark

Palabra de señalización

Danger

Frases de peligro

hazcat

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Órganos de actuación

Central nervous system

storage_class_code

3 - Flammable liquids

WGK Alemania

WGK 1

Punto de inflamabilidad F

53.6 °F - closed cup

Punto de inflamabilidad C

12.0 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de Análisis

Certificado de origen

Zsuzsanna Rácz et al.
Nucleic acid therapeutics, 21(3), 215-224 (2011-07-14)
Short-interfering RNAs (siRNAs), key mediators of RNA interference comprise a promising therapeutic tool, although side effects such as interferon (IFN) response are still not perfectly understood. Further, delivery to target organs is a major challenge, possibly associated with side effects...
Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many...
Peter K Davidsen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 110(2), 309-317 (2010-10-30)
MicroRNAs (miRNA), small noncoding RNA molecules, may regulate protein synthesis, while resistance exercise training (RT) is an efficient strategy for stimulating muscle protein synthesis in vivo. However, RT increases muscle mass, with a very wide range of effectiveness in humans....
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, E-E (1989)
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as...
Protocolos
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
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Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
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Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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Contenido relacionado
Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology
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