Merck

K3502

Sigma-Aldrich

Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat

affinity isolated antibody, lyophilized powder

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Número MDL:
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

formulario

lyophilized powder

technique(s)

quantitative precipitin assay: suitable

temp. de almacenamiento

2-8°C

target post-translational modification

unmodified

Gene Information

human ... IGK@(50802)

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Este artículo
A3813A5175L1645
conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

lyophilized powder

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

quantitative precipitin assay: suitable

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:35,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

indirect ELISA: 1:2,500

Descripción general

Anti-Human κ Light Chain (bound and free) antibody is obtained from goat antiserum. Immunospecific purification removes all goat serum proteins and immunoglobulins that do not bind to the κ light chain (bound and free). The antibody preparation is specific for κ light chains when tested against bound and free light chains. The product shows no reaction with bound or free λ light chains.

Inmunógeno

Purified human normal and myeloma κ light chain

Aplicación

Anti-Human κ Light Chain (Bound and Free)-FITC antibody was used for as secondary antibody in ELISA at a working antibody dilution of 1:200 using maize seed extracts. For ELISA using NSO/1 myeloma cells, HEK293 cells and mouse plasma, antibody concentration of 1-2 μg/ml was used. The antibody was also used to coat the electrode surface in the preparation of antiatrazine immunosensor.
Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat has been used in western blotting.

Forma física

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitución

Reconstitute with 0.135 M sodium chloride.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Journal of neural transmission (Vienna, Austria : 1996), 129(5-6), 545-555 (2022-04-03)
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Characterization of immunological interactions at an immunoelectrode by scanning electron microscopy
Ambrosi A et al
Electroanalysis, 19, 244-252 (2007)
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Intravascular delivery of broadly neutralizing antibodies (bnAbs) has shown promise for prevention and treatment of HIV infection. However, multiple IV administrations in geographic locations with poor accessibility to medical care have practical limitations. We have assessed the efficacy of plant-derived
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Proceedings of the National Academy of Sciences of the United States of America, 105(10), 3727-3732 (2008-03-05)
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