Merck

K4377

Sigma-Aldrich

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse

clone KP-53, ascites fluid

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Sinónimos:
Monoclonal Anti-Human Kappa Light Chain (Bound and Free)
Número MDL:
NACRES:
NA.46

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

secondary antibodies

clon

KP-53, monoclonal

contiene

15 mM sodium azide

technique(s)

indirect ELISA: 1:10,000

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

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Este artículo
R9010L6522A7164
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

affinity isolated antibody

clone

KP-53, monoclonal

clone

RT-39, monoclonal

clone

HP-6054, monoclonal

clone

polyclonal

technique(s)

indirect ELISA: 1:10,000

technique(s)

capture ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, indirect ELISA: 1:2,000, western blot: suitable using denatured and reduced normal serum or myeloma protein

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein, radioimmunoassay: suitable, western blot: suitable

technique(s)

direct ELISA: 1:1,000

isotype

IgG1

isotype

IgG1

isotype

IgG2a

isotype

-

Descripción general

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Especificidad

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Aplicación

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Forma física

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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25G
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Chloe L Stoyle et al.
The Biochemical journal, 474(18), 3179-3188 (2017-08-09)
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence.
Heterogeneity of aberrant immunoglobulin expression in cancer cells
Hu D, et al.
Cellular & Molecular Immunology, 8(6), 479-479 (2011)
Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
Zheng H, et al.
Molecular Immunology, 44(9), 2221-2227 (2007)
Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes
McBRIDE OW, et al.
The Journal of Experimental Medicine, 155(5), 1480-1490 (1982)
Dorothee Winterberg et al.
Journal of clinical medicine, 10(12) (2021-07-03)
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most frequent malignancy in children and also occurs in adulthood. Despite high cure rates, BCP-ALL chemotherapy can be highly toxic. This type of toxicity can most likely be reduced by antibody-based immunotherapy

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