L0159

Sigma-Aldrich

Anti-Luciferase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:
Luciferase Antibody - Anti-Luciferase antibody produced in rabbit, Anti Luciferase Antibody, Firefly Luciferase Antibody, Luciferase Antibody
Número MDL:
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

200

forma del anticuerpo

IgG fraction of antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

envase

antibody small pack of 25 μL

aplicaciones

indirect immunofluorescence: 1:500-1:1,000 using eukaryotic cells transfected with a plasmid bearing the luciferase gene
western blot: 1:10,000 using purified Luciferase

conjugado

unconjugated

enviado en

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Analysis of gene expression is commonly assayed by transient transfection. These systems are usually based on the use of fusion genes which are inserted into cells, and expression of the gene is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions is presumed to reflect the ability of the sequences studied to direct or promote transcription.
Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity.

Especificidad

The antibody identifies recombinant luciferase in eukaryotic cells transfected with a plasmid bearing the luciferase gene.

Inmunógeno

firefly (Photinus pyralis) luciferase.

Aplicación

Anti-Luciferase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Isabel Ml Saur et al.
eLife, 8 (2019-02-20)
Nucleotide-binding domain and leucine-rich repeat (NLR)-containing proteins in plants and animals mediate intracellular pathogen sensing. Plant NLRs typically detect strain-specific pathogen effectors and trigger immune responses often linked to localized host cell death. The barley Mla disease resistance locus has...
Marco Chiabudini et al.
Molecular and cellular biology, 32(23), 4769-4779 (2012-09-26)
When a polyadenylated nonstop transcript is fully translated, a complex consisting of the ribosome, the nonstop mRNA, and the C-terminally polylysine-tagged protein is generated. In Saccharomyces cerevisiae, a 3-step quality control system prevents formation of such dead-end complexes. Nonstop mRNA...
Intraductal delivery of adenoviruses targets pancreatic tumors in transgenic Ela-myc mice and orthotopic xenografts
Jose A, et al.
Testing, 4(1), 94-94 (2013)
Ayguen Sahin et al.
PloS one, 13(7), e0199414-e0199414 (2018-07-06)
Glioblastoma multiforme (GBM) is the most aggressive and deadly form of adult brain cancer. Despite of many attempts to identify potential therapies for this disease, including promising cancer immunotherapy approaches, it remains incurable. To address the need of improved persistence...
Anabel José et al.
Oncotarget, 4(1), 94-105 (2013-01-19)
Gene-based anticancer therapies delivered by adenoviruses are limited by the poor viral distribution into the tumor. In the current work we have explored the feasibility of targeting pancreatic tumors through a loco-regional route. We have taken advantage of the ductal...

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