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Merck

L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Sinónimos:

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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Número CAS:
9054-63-1
Número CE:
3.4.11.2 (BRENDA, IUBMB)
Número CE:
232-942-5
Número MDL:
MFCD00131501
Código UNSPSC:
12352204
NACRES:
NA.54

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origen biológico

Porcine kidney

Nivel de calidad

200

tpo

Type VI-S

Formulario

lyophilized powder

actividad específica

≥12 units/mg protein (biuret)

mol peso

320 kDa

composición

Protein, 30-70%

temp. de almacenamiento

−20°C

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Este artículo
P6675L5006164598
Leucine Aminopeptidase, microsomal from porcine kidney Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Sigma-Aldrich

L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Prolidase from porcine kidney lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

P6675

Prolidase from porcine kidney

Leucine Aminopeptidase, microsomal from porcine kidney Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

Sigma-Aldrich

L5006

Leucine Aminopeptidase, microsomal from porcine kidney

Aminopeptidase M, Porcine Kidney

Sigma-Aldrich

164598

Aminopeptidase M, Porcine Kidney

biological source

Porcine kidney

biological source

-

biological source

-

biological source

Porcine kidney

specific activity

≥12 units/mg protein (biuret)

specific activity

≥100 units/mg protein

specific activity

10-40 units/mg protein (Bradford)

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

suspension (crystalline)

mol wt

320 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Descripción general

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol.[1] It contains zinc in its structure and belongs to the M1 and M17 peptidase families.[2] LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.[3]

Aplicación

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2[4] and in the inhibition kinetic studies with APN inhibitor.[5][6]

Acciones bioquímicas o fisiológicas

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal.[1] An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy.[2] Porcine LAP hydrolyzes glycine and arginine substrates.[3]

Forma física

Contains primarily phosphate buffer salts, pH 7.0

Otras notas

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

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Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000313108
0000313107
0000313108
0000313107
0000280242

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Activity screening and structure-activity relationship of the hit compounds targeting APN/CD13
Wang X, et al.
Fundamental & Clinical Pharmacology, 25(2), 217-228 (2011)
Refolding of hexameric porcine leucine aminopeptidase using a cationic detergent and dextrin-10 as artificial chaperones
Laslo AC, et al.
Journal of Biotechnology, 140(3-4), 162-168 (2009)
The effect of different species Aminopeptidase N Structure on the activity screening of Aminopeptidase N Inhibitor
Wang X, et al.
Biological & Pharmaceutical Bulletin, 33(10), 1658-1665 (2010)
Novel and highly sensitive fluorescent assay for leucine aminopeptidases
Huang H, et al.
Analytical biochemistry, 391(1), 11-16 (2009)
Leucine aminopeptidases: diversity in structure and function
Matsui M, et al.
Biological Chemistry, 387(12), 1535-1544 (2006)
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Preguntas

1–2 de 2 Preguntas  

  1. I would like to know the optimum solvent for dissolving, storing and using this reagent (Cat # L6007-25UN).

    1 respuesta

    1. According to the Enzymatic Assay for Leucine Aminopeptidase, Microsomal Enzyme Solution, prepare a solution containing 0.10 - 0.15 unit/ml of Leucine Aminopeptidase, Microsomal in cold deionized water immediately before use.

      More information can be found at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/356/218/l6007enz.pdf.

      This formulation contains phosphate buffer salts. For reconstitution, storage, and use of this product, 50 mM Sodium Phosphate Buffer, pH7.2, is a suitable solvent.

      ¿Le ha resultado útil?

  2. I recently purchased Leucine Aminopeptidase from porcine kidney Cat # L6007-25UN. I'd like to inquire how many mgs of protein is in one vial.

    1 respuesta

    1. The milligrams of protein per vial will depend on the units/mg protein defined on the product's certificate of analysis. For example, lot SLCM3898 reports specific activity of 19 units/mg protein. This results in approximately 1.3 mg of protein per vial for this particular lot. The protein content of this lot of material is reported at 61%, therefore the total mass of solid material in the 25UN vial will total around 2.2 mg.

      (Link to certificate of analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L6007-BULKSLCM3898.pdf)

      ¿Le ha resultado útil?

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