Merck

L6522

Sigma-Aldrich

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

clone HP-6054, ascites fluid

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Sinónimos:
Monoclonal Anti-Human Lambda Light Chains (Bound and Free)
Número MDL:
NACRES:
NA.46

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

secondary antibodies

clon

HP-6054, monoclonal

contiene

15 mM sodium azide

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein
radioimmunoassay: suitable
western blot: suitable

isotipo

IgG2a

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

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Este artículo
R9010A5175L1645
conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

HP-6054, monoclonal

clone

RT-39, monoclonal

clone

polyclonal

clone

polyclonal

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein, radioimmunoassay: suitable, western blot: suitable

technique(s)

capture ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, indirect ELISA: 1:2,000, western blot: suitable using denatured and reduced normal serum or myeloma protein

technique(s)

direct ELISA: 1:35,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

indirect ELISA: 1:2,500

isotype

IgG2a

isotype

IgG1

isotype

-

isotype

-

Descripción general

Monoclonal Anti-Human Lambda Light Chains (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Especificidad

The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains.

Inmunógeno

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Aplicación

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse has been used:
  • in immunohistochemistry
  • in enzyme-linked immunosorbent assay (ELISA) assay
  • in immunohistology
  • in radioimmunoassay (RIA)
  • in precipitation assays
  • in immunoblotting
  • as capture antibodies in quantitative ELISA

Acciones bioquímicas o fisiológicas

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Primary lymphoma of the central nervous system: a clinical-pathological and immunohistochemical study of ten autopsy cases
Costa H, et al.
Arquivos de Neuro-Psiquiatria, 64(4), 976-982 (2006)
Patrizia Circelli et al.
Bioengineered bugs, 1(3), 221-224 (2011-02-18)
We have recently described an efficient transient expression system mediated by Agrobacterium tumefaciens for the production of HIV-1 Nef protein in Nicotiana benthamiana plants. In order to enhance the yield of recombinant protein we assayed the effect of three gene-silencing
Maria Elena Villani et al.
Plant biotechnology journal, 7(1), 59-72 (2008-09-17)
The aims of this work were to obtain a human antibody against the tumour-associated antigen tenascin-C (TNC) and to compare the yield and quality of plant-produced antibody in either stable transgenics or using a transient expression system. To this end
A human immunoglobulin lambda locus is similarly well expressed in mice and humans
Popov AV, et al.
The Journal of Experimental Medicine, 189(10), 1611-1620 (1999)
Jerry A Katzmann et al.
Clinical chemistry, 48(9), 1437-1444 (2002-08-27)
The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more

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