M218

Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α4 Subunit) antibody produced in rat

~1 mg/mL, clone mAb 299, purified immunoglobulin, buffered aqueous solution

Número MDL:
NACRES:
NA.41

Nivel de calidad

100

origen biológico

rat

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

mAb 299, monoclonal

formulario

buffered aqueous solution

species reactivity

human, rat, chicken

concentración

~1 mg/mL

aplicaciones

immunohistochemistry (frozen sections): 1:800
radioimmunoassay: suitable

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... CHRNA4(1137)
rat ... Chrna4(25590)

Especificidad

Binds to native and denatured α4 AchR. May be used to quantitate α4 AchR′s on the cell surface. The epitope is located on the extracellular surface of the α4 subunit of the AchR.

Inmunógeno

nicotinic acetylcholine receptor (AchR) from the rat brain.

Envase

100 μL in glass insert

Forma física

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Información legal

Sold with permission of the Salk Institute

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

H Nakayama et al.
Brain research. Molecular brain research, 32(2), 321-328 (1995-09-01)
Localization of nicotinic acetylcholine receptor (nAChR) alpha 4 subunits was investigated in rat cerebral cortex using a monoclonal antibody against alpha 4 subunits. The antibody depleted more than 70% of the [3H]methylcarbamylcholine choline binding activity of the solubilized membrane fraction....
X Peng et al.
Molecular pharmacology, 46(3), 523-530 (1994-09-01)
Chronic nicotine exposure in tobacco smokers or experimental animals is known to cause an increase in brain binding sites for nicotine. It has been proposed that this is an adaptive response of neurons to accumulation of chronically desensitized receptors. Acetylcholine...
P J Whiting et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 8(9), 3395-3404 (1988-09-01)
Neuronal acetylcholine receptors (AChRs), which bind nicotine with high affinity but do not bind alpha-bungarotoxin (alpha Bgt), have recently been immunoaffinity-purified from chicken (Whiting and Lindstrom, 1986a) and rat (Whiting and Lindstrom, 1987a) brain using monoclonal antibodies (mAbs). Here we...
V V Pollock et al.
Neuroscience, 158(4), 1311-1325 (2008-12-23)
Neuronal nicotinic receptor alpha4 subunits associated with nicotinic alpha4beta2 receptors are phosphorylated by cyclic AMP-dependent protein kinase (PKA) and protein kinase C (PKC), but the stages of receptor formation during which phosphorylation occurs and the functional consequences of kinase activation...
Lindstrom, J.
Neurotransmissions, 12 (1996)

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