M4403

Sigma-Aldrich

Monoclonal Anti-MAP2 antibody produced in mouse

clone HM-2, ascites fluid

Sinónimos:
Map2 Antibody, MAP2 Antibody - Monoclonal Anti-MAP2 antibody produced in mouse
Número MDL:
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

HM-2, monoclonal

mol peso

antigen ~280 kDa

contiene

15 mM sodium azide

species reactivity

rat, mouse, chicken, human, bovine, quail

aplicaciones

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:1,000 using a fresh total rat brain extract or an enriched microtubule protein preparation

isotipo

IgG1

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

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Descripción general

Microtubule-associated protein 2 (MAP2) is a neuronal protein. It belongs to the MAP2/Tau family. This gene is located to human chromosome 2q34-q35. MAP2 is present in neurons. MAP2 is the major microtubule-associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70kDa (MAP2c).

Especificidad

Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

Inmunógeno

rat brain microtubule-associated proteins (MAPs).

Aplicación

Monoclonal Anti-MAP2 (2a+2b) antibody has been used in immunohistochemistry, immunostaining and western blotting.

Acciones bioquímicas o fisiológicas

Microtubule-associated protein 2 (MAP2) interacts with microtubules to maintain the structure of dendrites. It plays a major role in supporting the actin cytoskeleton in spines, binding and nucleating filamentous actin (f-actin) to modulate spine morphology.

Forma física

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Alemania

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

GABA and histogenesis in fetal and neonatal mouse brain lacking both the isoforms of glutamic acid decarboxylase.
Ji F, et al.
Neuroscience Research, 33(3), 187-194 (1999)
Neural differentiation of mouse embryonic stem cells grown in monolayer.
Pachernik J, et al.
Reproduction, Nutrition, Development, 42(4), 317-326 (2002)
An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative.
Evers P and Uylings HBM
Journal of Neuroscience Methods, 72(2), 197-207 (1997)
Loss of microtubule-associated protein 2 immunoreactivity linked to dendritic spine loss in schizophrenia.
Shelton MA, et al.
Biological Psychiatry, 78(6), 374-385 (2015)
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L and Halpain S
Genome Biology, 6(1), 204-204 (2005)

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