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M5899

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) antibody produced in goat

whole antiserum

Número MDL:
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

whole antiserum

antibody product type

secondary antibodies

clon

polyclonal

contiene

15 mM sodium azide

technique(s)

indirect ELISA: 1:70,000
quantitative precipitin assay: 3.2-4.8 mg/mL

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Especificidad

The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) against normal mouse serum and purified mouse IgG.

Aplicación

Anti-Mouse IgG (whole molecule) antibody produced in goat has been used as secondary antibody in:
  • immunoblotting
  • immunohistochemistry
  • proteomic microarrays
Anti-Mouse IgG (whole molecule) antibody produced in goat has been used in:
  • the preparation of immunochromatography strips for magneto-enzyme lateral flow immunoassay
  • immunochromatography
  • MYCN DNA-binding analysis (ChIP-seq)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Acciones bioquímicas o fisiológicas

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Calidad

The antiserum has been treated to remove lipoproteins.

Forma física

Supplied as a liquid containing 15 mM sodium azide as preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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