Merck
All Photos(3)

MAK102

Sigma-Aldrich

PEP Colorimetric/Fluorometric Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):
Phospho(enol)pyruvate Colorimetric/Fluorometric Assay Kit
Número de EC:
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

temp. de almacenamiento

−20°C

Related Categories

Descripción general

Phospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Aplicación

PEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extracts

Principio

PEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PEP present.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

188.6 °F - closed cup

Punto de inflamabilidad C

87 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service