Merck
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N2765

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

tablet

Synonym(s):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein:
5324661
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.83

formulario

tablet

Nivel de calidad

300

solubilidad

water, high purity: 0.25 tablet/mL, clear, colorless

temp. de almacenamiento

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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Descripción general

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Aplicación

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
  • as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
  • in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample

Cantidad

Contains 20 mg substrate per tablet

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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