N3877

Potassium 4-nitrophenyl sulfate

Name: Potassium 4-nitrophenyl sulfate
Brand: SIGMA

sulfatase substrate, chromogenic, ≥98% (TLC), powder

Sinónimos:

4-Nitrophenyl sulfate potassium salt

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Fórmula lineal:

NO₂C₆H₄OSO₂OK

Número CAS:

6217-68-1

Peso molecular:

257.26

Beilstein:

3786392

Número CE:

228-288-5

Número MDL:

MFCD00007466

Código UNSPSC:

12352204

ID de la sustancia en PubChem:

24897664

NACRES:

NA.32

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Nombre del producto

Potassium 4-nitrophenyl sulfate, sulfatase substrate

Nivel de calidad

200

Ensayo

≥98% (TLC)

Formulario

powder

mp

246-250 °C (lit.)

solubilidad

water: 50 mg/mL, clear, colorless to very faintly greenish-yellow (to Very Light Yellow)

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

cadena SMILES

[K+].[O-][N+](=O)c1ccc(OS([O-])(=O)=O)cc1

InChI

1S/C6H5NO6S.K/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;/h1-4H,(H,10,11,12);/q;+1/p-1

Clave InChI

BITVAZYUWRLLCN-UHFFFAOYSA-M

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Este artículo	B1379	N3002	N0252
Sigma-Aldrich N3877 Potassium 4-nitrophenyl sulfate	Sigma-Aldrich B1379 5-Bromo-4-chloro-3-indolyl sulfate potassium salt	Sigma-Aldrich N3002 Bis(p-nitrophenyl) phosphate sodium salt	Sigma-Aldrich N0252 4-Nitrophenyl decanoate
assay ≥98% (TLC)	assay ≥98% (TLC)	assay ≥99% (TLC)	assay ≥98% (TLC)
solubility water: 50 mg/mL, clear, colorless to very faintly greenish-yellow (to Very Light Yellow)	solubility water: 50 mg/mL, clear to slightly hazy	solubility water: 20 mg/mL, clear to very slightly hazy	solubility chloroform: soluble 100 mg/mL, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form powder	form powder
shipped in wet ice	shipped in -	shipped in -	shipped in -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Descripción general

Chromogenic sulfatase substrate.

Aplicación

Potassium 4-nitrophenyl sulfate has been used:

as a substrate to measure arylsulfatase activity in cell-free coelomic fluid[1]
as a substrate for p-nitrophenyl glycoside-based enzyme assay[2]
to inhibit arylsulfatase[3]
as an inorganic analog of organic aryl ester sulphate[4]

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

0000505608

0000502676

0000485542

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Nucleotide binding and sulfation catalyzed by phenol sulfotransferase.

E S Lin et al.

Biochemical and biophysical research communications, 271(3), 818-822 (2000-05-18)

The sulfation of a nucleotide is an indispensable step for the sulfuryl group transfer in a biological system. The product and cosubstrate of sulfotransferase in physiological condition are adenosine 3',5'-bisphosphate (PAP) and 3'-phospho adenosine 5'-phosphosulfate (PAPS), respectively. We find that

A continuous assay for the spectrophotometric analysis of sulfotransferases using aryl sulfotransferase IV.

M D Burkart et al.

Analytical biochemistry, 274(1), 131-137 (1999-10-21)

We have developed a continuous spectrophotometric coupled-enzyme assay for sulfotransferase activity. This assay is based on the regeneration of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) from the desulfated 3'-phosphoadenosine-5'-phosphate (PAP) by a recombinant aryl sulfotransferase using p-nitrophenyl sulfate as the sulfate donor and visible

Theoretical comparison of p-nitrophenyl phosphate and sulfate hydrolysis in aqueous solution: implications for enzyme-catalyzed sulfuryl transfer.

Shina Caroline Lynn Kamerlin

The Journal of organic chemistry, 76(22), 9228-9238 (2011-10-11)

Both phosphoryl and sulfuryl transfers are ubiquitous in biology, being involved in a wide range of processes, ranging from cell division to apoptosis. Additionally, it is becoming increasingly clear that enzymes that can catalyze phosphoryl transfer can often cross-catalyze sulfuryl

A dietary fiber-deprived gut microbiota degrades the colonic mucus barrier and enhances pathogen susceptibility

Desai MS, et al.

Cell, 167(5), 1339-1353 (2016)

Hepatic uptake of p-nitrophenyl sulfate by transporter that acetaminophen sulfate shares for uptake: sulfate moiety as a vector for metabolite transport.

N Sakuma-Sawada et al.

Research communications in molecular pathology and pharmacology, 97(2), 131-138 (1997-08-01)

Hepatic uptake of the sulfate conjugate of p-nitrophenol (p-NPsul) has been studied. Uptake clearance of p-NPsul by isolated rat hepatocytes was dependent on p-NPsul concentration, suggesting carrier-mediated transport. The uptake of p-NPsul by isolated rat hepatocytes was inhibited by acetaminophen

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