N9913

Sigma-Aldrich

NEK7, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Nivel de calidad

200

recombinante

expressed in baculovirus infected Sf9 cells

línea de producto

PRECISIO® Kinase

ensayo

≥70% (SDS-PAGE)

formulario

buffered aqueous glycerol solution

specific activity

188-254 nmol/min·mg

mol peso

~63 kDa

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... NEK7(140609)

Acciones bioquímicas o fisiológicas

NEK7 is a member of the NIMA family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, NEK7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that NEK7 is cytoplasmic and located on chromosome 1. The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412, was purified from rat liver and identified as NEK7.NEK7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.

Forma física

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Información legal

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

M Kandli et al.
Genomics, 68(2), 187-196 (2000-08-31)
Entrance and exit from mitosis in Aspergillus nidulans require activation and proteolysis, respectively, of the NIMA (never in mitosis, gene A) serine/threonine kinase. Four different NIMA-related kinases were reported in mammals (Nek1-4), but none of them has been shown to...
C Belham et al.
Current biology : CB, 11(15), 1155-1167 (2001-08-23)
The p70 S6 kinase, like several other AGC family kinases, requires for activation the concurrent phosphorylation of a site on its activation loop and a site carboxyterminal to the catalytic domain, situated in a hydrophobic motif site FXXFS/TF/Y, e.g.,Thr412 in...

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