Merck
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P0067

Sigma-Aldrich

Anti-p62/SQSTM1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Sequestosome 1, Anti-Ubiquitin-binding p62
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~62 kDa

species reactivity

human, rat, mouse

envase

antibody small pack of 25 μL

concentración

~1 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using lysate of NIH-3T3 cells
indirect immunofluorescence: 1-2 μg/mL using human A549 cells
western blot: 1-2 μg/mL using whole extracts of rat PC12 cells

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
rat ... Sqstm1(113894)

Related Categories

Descripción general

p62 or SQSTM1 (sequestosome 1) is a multifunctional, multi-domain adaptor protein which resides at the autophagosome membranes. The human p62 gene is mapped to human chromosome 5. The protein has 440 amino acids.[1] This protein is conserved across metazoans, but not plant and fungi.[2] It is composed of Phox1 and Bem1p (PB1) domain, a zinc finger (ZZ) domain, two nuclear localization signals, a tumor necrosis factor receptor-associated factor 6 (TRAF6) binding domain, a nuclear export signal, an LC3-interacting region (LIR), a Keap1-interacting region (KIR) and a ubiquitin-associated (UBA) domain. It is predominantly present in cytoplasm, but also shows expression in nucleus, autophagosomes and lysosomes.[3][4][5]
Anti-p62/SQSTM1 is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids of human p62/SQSTM1 (GeneID: 8878), conjugated to KLH. The corresponding sequence is identical in rat and mouse. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Especificidad

Anti-p62/SQSTM1 recognizes human, rat, and mouse p62/SQSTM1. The antibody may be used in various immunochemical techniques including immunoblotting (~ 62 kDa), immunoprecipitation, and indirect immunofluorescence. Detection of the p62/SQSTM1 band by immunoblotting is specifically inhibited by the immunizing peptide.

Inmunógeno

Synthetic peptide corresponding to amino acids of human p62/SQSTM1, conjugated to KLH. The corresponding protein sequence is identical in rat and mouse.

Aplicación

Anti-p62/SQSTM1 antibody produced in rabbit has been used in:
  • western blotting[6][7][8]
  • immunoprecipitation in human cell lines[9]
  • Immunohistochemistry prostatectomy specimens[10]

Acciones bioquímicas o fisiológicas

p62 or SQSTM1 (sequestosome 1) plays a role in the catabolic metabolism of molecules involved in NF-κB (nuclear factor), mTOR (mammalian target of rapamycin), MAPK (mitogen activated protein kinase), and therefore, is involved in cell cycle and apoptosis. It is thought to function as an oncogene, and its accumulation has been associated with poor prognosis. It is accumulated in cell with defective autophagy, and its cytosolic accumulation is linked with poor prognosis in prostate cancer.[3] It is responsible for the activation of mammalian target of rapamycin complex 1 (mTORC1) by functioning as an adaptor for mTORC1 lysosomal membrane.[5] During Dengue virus (DENV) infection, the expression of p62 protein is reduced due to proteasomal degradation, and stable expression of p62 results in decreased DENV replication.[4] p62 is an autophagy marker, and its accumulation is linked with aberration in autophagic degradation or inhibition of autophagy.[5]
Mutations in this gene result in sporadic and familial Paget disease of bone.[11] p62 is commonly found in inclusion bodies containing polyubiquitinated protein aggregates, that accumulate in several degenerative diseases. Autophagy is involved in cellular clearance of these protein aggregates.[12] Autophagy plays an essential role in cellular differentiation, cell death, and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders, and pathogen infections.[13]

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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