P0074

Sigma-Aldrich

Anti-PERK (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:
Anti-E2AK3, Anti-HsPEK, Anti-EIF2AK3, Anti-WRS, Anti-PEK, Anti-Pancreatic eIF2α kinase, Anti-PRKR-like endoplasmic reticulum kinase
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

200

forma del anticuerpo

IgG fraction of antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~150 kDa

species reactivity

human (predicted), mouse, rat

aplicaciones

western blot: 1:500-1:1,000 using whole cell lysate of HEK-293T expressing PERK cells

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... Eif2ak3(13666)
mouse ... Eif2ak3(13666)
rat ... Eif2ak3(29702)

Descripción general

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) belongs to a family of eukaryotic initiation factor 2 α (eIF2α) kinases. It is a transmembrane kinase that is highly expressed in the pancreas. PERK contains two main domains: a kinase domain and an N-terminal domain like inositol- requiring enzyme-1 (IRE1), a protein involved in the unfolded protein response (UPR).
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is one of the three mammalian unfolded protein response (UPR) transducers. It is a highly conserved eukaryotic initiation factor 2 α (eIF2α) kinase. The gene encoding this protein is localized on human chromosome 2p11.2.

Especificidad

Anti-PERK C-terminal specifically recognizes PERK.

Inmunógeno

synthetic peptide corresponding to amino acids 1078-1092 of mouse PERK, conjugated to KLH. This sequence is identical in rat and differs by one amino acid in human.

Aplicación

Anti-PERK (C-terminal) antibody produced in rabbit has been used for western blotting and ERK (extracellular-signal-regulated kinase) phosphorylation assays.

Acciones bioquímicas o fisiológicas

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an endoplasmic reticulum (ER) stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation and osteopenia.
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) responds to distinct cellular stress signals.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 2

Role of the C-terminal di-leucine motif of 5-HT1A and 5-HT1B serotonin receptors in plasma membrane targeting
Damien Carrel
Journal of Cell Science (2006)
PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia.
Radford H
Acta Neuropathologica (2015)
The eIF2a kinase PERK limits the expression of hippocampal metabotropic glutamate receptor-dependent long-term depression.
Trinh MA
Learning & Memory (2014)
Perk gene dosage regulates glucose homeostasis by modulating pancreatic ?-cell functions.
Perk gene dosage regulates glucose homeostasis by modulating pancreatic ?-cell functions.
Wang R
PLoS ONE (2014)
Identification of common variants influencing risk of the tauopathy Progressive Supranuclear Palsy
Gunter U
Nature Genetics (2011)

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