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P3910

Sigma-Aldrich

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Sinónimos:
Proteinase from Bacillus licheniformis, Subtilisin A
Número de CAS:
Comisión internacional de enzimas:
Número de EC:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.84

formulario

lyophilized powder

Nivel de calidad

100

calidad

for use in Total Dietary Fiber Assay, TDF-100A

mol peso

27 kDa

application(s)

food and beverages

enviado en

wet ice

temp. de almacenamiento

2-8°C

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Especificidad

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Aplicación

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Envase

500 mg in poly bottle

Acciones bioquímicas o fisiológicas

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Propiedades físicas

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Definición de unidad

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de Análisis

Certificado de origen

Micaela Vannini et al.
Polymers, 13(7) (2021-04-04)
With the aim to fully exploit the by-products obtained after the industrial extraction of starch from sweet potatoes, a cascading approach was developed to extract high-value molecules, such as proteins and pectins, and to valorize the solid fraction, rich in
Lisete Paiva et al.
Marine drugs, 16(8) (2018-07-28)
This study evaluates, for the first time, the seasonal (winter and summer) and geographical (São Miguel⁻SMG and Santa Maria⁻SMA Islands) variability of Fucus spiralis (Fs) biochemical composition (dry weight basis) and antioxidant properties. Protein and carbohydrates presented higher values in
Yong Zhuo et al.
Journal of animal science and biotechnology, 11, 47-47 (2020-05-20)
This study aimed to investigate the impacts of guar gum and cellulose as the source of dietary fiber during gestation on the reproductive performance of sows. A total of 210 sows (parities 3-6) were randomly allocated into six diets (n = 35)
Derya Y Koseoglu-Imer et al.
Colloids and surfaces. B, Biointerfaces, 92, 334-339 (2012-01-06)
In this study, the surface of cellulose acetate (CA) ultrafiltration membrane was activated with serine protease (Savinase) enzyme to reduce protein fouling. Enzyme molecules were covalently immobilized with glutaraldehyde (cross-linking agent) onto the surface of CA membranes. The membrane activation
P Nedkov et al.
Biological chemistry Hoppe-Seyler, 366(4), 421-430 (1985-04-01)
The complete amino-acid sequence of subtilisin DY, an extracellular alkaline proteinase produced by Bacillus subtilis strain DY was determined. This included automated sequence analysis of the whole molecule and its large fragments such as tryptic peptides obtained from the inactivated

Protocolos

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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