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Monoclonal Anti-Rat IgG2a antibody produced in mouse

clone R2A-2, ascites fluid

Número MDL:

Nivel de calidad


origen biológico




forma del anticuerpo

ascites fluid

antibody product type

secondary antibodies


R2A-2, monoclonal


15 mM sodium azide


capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:5,000
western blot: suitable



enviado en

dry ice

temp. de almacenamiento


Categorías relacionadas

Descripción general

Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.
Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.


Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.


Purified rat IgG


Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • slot blot immunoassay
  • immunoblotting
  • dot blot
  • immunocytochemistry

Acciones bioquímicas o fisiológicas

Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.

Forma física

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 0-5 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids



Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

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Certificado de origen

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Fichas técnicas

Presupuestos y pedidos

Keiichi Itoi et al.
Endocrinology, 155(10), 4054-4060 (2014-07-25)
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H Bazin et al.
European journal of immunology, 4(1), 44-48 (1974-01-01)
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Chungang Feng et al.
PLoS genetics, 10(8), e1004576-e1004576 (2014-08-29)
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