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R0761

Sigma-Aldrich

Monoclonal Anti-Rat IgG2a antibody produced in mouse

clone R2A-2, ascites fluid

Número MDL:
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

secondary antibodies

clon

R2A-2, monoclonal

contiene

15 mM sodium azide

technique(s)

capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:5,000
western blot: suitable

isotipo

IgG1

enviado en

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.
Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.

Especificidad

Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.

Inmunógeno

Purified rat IgG

Aplicación

Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • slot blot immunoassay
  • immunoblotting
  • dot blot
  • immunocytochemistry

Acciones bioquímicas o fisiológicas

Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.

Forma física

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 0-5 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Fichas técnicas

Presupuestos y pedidos

Keiichi Itoi et al.
Endocrinology, 155(10), 4054-4060 (2014-07-25)
Corticotropin-releasing factor (CRF) is the key regulator of the hypothalamic-pituitary-adrenal axis. CRF neurons cannot be distinguished morphologically from other neuroendocrine neurons in the paraventricular nucleus of the hypothalamus (PVH) without immunostaining. Thus, we generated a knock-in mouse that expresses modified
Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
H Bazin et al.
European journal of immunology, 4(1), 44-48 (1974-01-01)
Ryuta Uraki et al.
Vaccine, 32(41), 5295-5300 (2014-08-12)
Because vaccination is an effective means to protect humans from influenza viruses, extensive efforts have been made to develop not only new vaccines, but also for new adjuvants to enhance the efficacy of existing inactivated vaccines. Here, we examined the
Yang Zhao et al.
Stem cells and development, 23(18), 2143-2155 (2013-11-19)
Embryonic stem (ES) cells are useful for elucidating the molecular mechanisms of cell fate decision in the early development of mammals. It has been shown that aggregate culture of ES cells efficiently induces neuroectoderm differentiation. However, the molecular mechanism that
Chungang Feng et al.
PLoS genetics, 10(8), e1004576-e1004576 (2014-08-29)
Silky-feather has been selected and fixed in some breeds due to its unique appearance. This phenotype is caused by a single recessive gene (hookless, h). Here we map the silky-feather locus to chromosome 3 by linkage analysis and subsequently fine-map

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