Merck
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R0883

Sigma-Aldrich

RPMI-1640 Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Roswell Park Memorial Institute 1640 medium
Número MDL:
NACRES:
NA.75

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

technique(s)

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes

enviado en

ambient

temp. de almacenamiento

2-8°C

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Descripción general

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Aplicación

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)

Reconstitución

Supplement with 0.3 g/L L-glutamine.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Formulation

SDS

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