R1008

Sigma-Aldrich

Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse

clone RG-96, ascites fluid

Sinónimos:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

ascites fluid

antibody product type

secondary antibodies

clon

RG-96, monoclonal

contiene

15 mM sodium azide

species reactivity

rabbit

should not react with

chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine

aplicaciones

dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:2,000
western blot: suitable

isotipo

IgG1

conjugado

unconjugated

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Especificidad

Monoclonal Anti-Rabbit IgG is specific for an epitope located on the heavy (γ) chain of rabbit IgG, in an immunoblot of denatured, reduced rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, guinea pig, horse, human, pig, rat or sheep.

Aplicación

Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • dot blots
  • immunohistology
  • western blotting
  • peptide mass fingerprinting (PMF)

Acciones bioquímicas o fisiológicas

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc region is involved in the development of therapeutic antibody.

Forma física

The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemania

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

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