R2655

Sigma-Aldrich

Anti-Rabbit IgG, Native antibody, Mouse monoclonal

clone RabT-50, purified from hybridoma cell culture

NACRES:
NA.46

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

secondary antibodies

clon

RabT-50, monoclonal

formulario

buffered aqueous solution

species reactivity

rabbit

should not react with

horse, monkey, cat, human, goat, pig, bovine, rat, chicken

concentración

~1 mg/mL

aplicaciones

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane

isotipo

IgG1

conjugado

unconjugated

enviado en

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody.

Especificidad

Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs.

Inmunógeno

Enriched fraction of rabbit IgG light chain.

Aplicación

Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)

Acciones bioquímicas o fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de Análisis

Certificado de origen

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