R3155

Sigma-Aldrich

Monoclonal Anti-Rabbit IgG, Native−Peroxidase antibody produced in mouse

clone RabT-50, purified from hybridoma cell culture

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified immunoglobulin

antibody product type

secondary antibodies

clon

RabT-50, monoclonal

formulario

buffered aqueous solution

species reactivity

rabbit

should not react with

human, pig, rat, goat, monkey, bovine, horse, chicken, cat

concentración

2.5-5.0 mg/mL (antibody)

aplicaciones

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody
western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.

isotipo

IgG1

conjugado

peroxidase conjugate

enviado en

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.

Especificidad

Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Inmunógeno

Enriched fraction of rabbit IgG light chain.

Aplicación

Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • western blotting

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

hazcat

Skin Sens. 1

storage_class_code

12 - Non Combustible Liquids

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

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