S1073

Sigma-Aldrich

Anti-STUB1/CHIP antibody, mouse monoclonal

clone ST21.55, purified from hybridoma cell culture

Sinónimos:
Anti-STIP1 homologous and box-containing protein 1 (STUB1), Anti-HSPABP2, Anti-Serologically defined colon cancer antigen 7, Anti-C terminus of HSC70-interacting protein (CHIP), Anti-Heat shock protein A binding protein 2 (c-terminal), Anti-UBOX1, Anti-NY-CO-7, Anti-SDCCAG7
Número MDL:
NACRES:
NA.41

Nivel de calidad

200

origen biológico

mouse

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

ST21.55, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~35 kDa

species reactivity

bovine, human, rat

concentración

~1.0 mg/mL

aplicaciones

indirect ELISA: suitable
western blot: 2.5-5 μg/mL using HeLa total cell extract.

isotipo

IgG2b

conjugado

unconjugated

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

bovine ... STUB1(504565)
human ... STUB1(10273)
mouse ... Stub1(56424)
rat ... Stub1(287155)

Descripción general

Monoclonal Anti-STUB1/CHIP (mouse IgG2b isotype) is derived from the hybridoma ST21.55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP. STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) consists of three functional domains: a tetratricopeptide repeat (TRP) at the amino terminus, a U- box domain at the C-terminus, and a highly charged region separating the two.
STUB1 (STIP1 homology and U-box containing protein 1) or CHIP (carboxyl terminus of Hsc70- interacting protein) is an E3 ubiquitin ligase, (2) that is located on human chromosome 16p13.3.

Inmunógeno

synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP.

Aplicación

Anti-STUB1/CHIP antibody, mouse monoclonal has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).

Acciones bioquímicas o fisiológicas

In lens epithelial cells, knocking down CHIP (carboxyl terminus of Hsc70- interacting protein) decreased the reaction of heat shock and potential of protein quality control. It plays a major role in neurodegeneration and also controls autophagic flux. It can deactivate NF-κB signaling and damage the capability of migration and invasion in gastric cancer cells.
STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) is such a cofactor, which interacts, among others, with Hsc70 and acts as a U-box-dependent E3 ubiquitin ligase. STUB1/CHIP can also act as a direct chaperone of p53, both under physiological and stress conditions.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

CHIP Knockdown Reduced Heat Shock Response and Protein Quality Control Capacity in Lens Epithelial Cells
Zhang W, et al.
Current Molecular Medicine, 15(7), 652-662 (2015)
CHIP (carboxyl terminus of Hsc70-interacting protein) promotes basal and geldanamycin-induced degradation of estrogen receptor-alpha
Fan M, et al.
Molecular Endocrinology, 19(12), 2901-2914 (2005)
Ataxia and hypogonadism caused by the loss of ubiquitin ligase activity of the U box protein CHIP
Shi CH, et al.
Human Molecular Genetics, 23(4), 1013-1024 (2013)
Chang-He Shi et al.
PLoS genetics, 14(9), e1007664-e1007664 (2018-09-18)
CHIP (carboxyl terminus of heat shock 70-interacting protein) has long been recognized as an active member of the cellular protein quality control system given the ability of CHIP to function as both a co-chaperone and ubiquitin ligase. We discovered a...
F Bertucci et al.
Oncogene, 27(40), 5359-5372 (2008-05-21)
Invasive ductal carcinomas (IDCs) and invasive lobular carcinomas (ILCs) are the two major pathological types of breast cancer. Epidemiological and histoclinical data suggest biological differences, but little is known about the molecular alterations involved in ILCs. We undertook a comparative...

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