S5761

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

• Synonym(s): Sodium hydrogen carbonate, soda bicarbonate
• Linear Formula: NaHCO₃
• CAS Number: 144-55-8
• Molecular Weight: 84.01
• Beilstein: 4153970
• Número de EC: 205-633-8
• Número MDL: MFCD00003528
• ID de la sustancia en PubChem: 24899673
• NACRES: NA.75

Properties

• grado: for molecular biology
• Nivel de calidad: 300
• Línea del producto: BioReagent
• ensayo: 99.5-100.5%
• formulario: powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• pH: 6.8-7.4
• pKa (25 °C): (1) 6.37, (2) 10.25 (carbonic acid)
• solubilidad: water: soluble 100 mg/mL
• densidad: 2.16 g/mL at 25 °C (lit.)
• SMILES string: [Na+].OC([O-])=O
• InChI: 1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
• InChI key: UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

Descripción general

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Aplicación

Sodium bicarbonate has been used:

• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
• as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
• as a component of medium used for cell culture of human embryonic kidney cells[4]
• as a component of Beltsville thawing solution used for the dilution of semen[5]

Envase

500 g in poly bottle

1 kg in poly bottle

5 kg in poly drum

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)