Merck
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S5761

Sigma-Aldrich

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Synonym(s):
Sodium hydrogen carbonate, soda bicarbonate
Linear Formula:
NaHCO3
CAS Number:
Molecular Weight:
84.01
Beilstein:
4153970
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.75

grado

for molecular biology

Nivel de calidad

300

Línea del producto

BioReagent

ensayo

99.5-100.5%

formulario

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

6.8-7.4

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubilidad

water: soluble 100 mg/mL

densidad

2.16 g/mL at 25 °C (lit.)

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Descripción general

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Aplicación

Sodium bicarbonate has been used:
  • as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
  • as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
  • as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
  • as a component of medium used for cell culture of human embryonic kidney cells[4]
  • as a component of Beltsville thawing solution used for the dilution of semen[5]

Envase

500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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