SAB4200670

Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal

clone NOQ7.5.4D, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM
• NACRES: NA.41

Properties

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clon: NOQ7.5.4D, monoclonal
• formulario: buffered aqueous solution
• species reactivity: sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine
• envase: antibody small pack of 25 μL
• concentración: ~1 mg/mL
• technique(s): immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate.; immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin^®-Peroxidase staining system.
• isotipo: IgG1
• enviado en: dry ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... MYH7(4625)

Description

Descripción general

Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle.

Inmunógeno

Myosin, purified from myofibrils isolated from human skeletal muscle,

Aplicación

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy.

Acciones bioquímicas o fisiológicas

Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion.

Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Información legal

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable