Merck
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SAB4700050

Sigma-Aldrich

Monoclonal Anti-CD3 antibody produced in mouse

clone APA1/1, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-CD3 (activation epitope)
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

100

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

APA1/1, monoclonal

formulario

buffered aqueous solution

species reactivity

mouse, human

concentración

1 mg/mL

technique(s)

flow cytometry: suitable

isotipo

IgG1

Nº de acceso NCBI

enviado en

wet ice

temp. de almacenamiento

2-8°C

Gene Information

human ... CD3(916)

Descripción general

CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes.
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Inmunógeno

Purified human CD3 proteins isolated from thymus

Aplicación

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Acciones bioquímicas o fisiológicas

T-cell receptor- CD3 (cluster of differentiation 3) complex including CD3ε, CD3γ and CD3δ, plays a vital role in inducing early metabolic events that lead to T cell activation. Mutation in CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

Características y beneficios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forma física

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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