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T4184

Sigma-Aldrich

Thrombopoietin from mouse

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):
TPO
CAS Number:
Número MDL:
NACRES:
NA.32

origen biológico

mouse

Nivel de calidad

200

recombinante

expressed in NSO cells

ensayo

>97% (SDS-PAGE)

formulario

lyophilized powder

potencia

2.1 ng/mL EC50

mol peso

~75 kDa by SDS-PAGE (as a result of glycosylation)
predicted mol wt 35 kDa

envase

pkg of 5 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurezas

endotoxin, tested

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

mouse ... Thpo(21832)

Aplicación

Thrombopoietin from mouse can be used in a genetic screening test for examining the role of somatic activating mutations in EPOR, MPL, or GCSFR genes in activating myeloproliferative disorders (MPD) in JAK2V617F-negative MPD patients.

Acciones bioquímicas o fisiológicas

Thrombopoeitin (TPO), also known as the ligand for the c-mpl proto-oncogene (Mpl-L) and as megakaryocyte colony stimulating factor (Meg-CSF), is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. Produced mainly by hepatocytes but also by kidney cells, TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved glycoprotein, showing approximately 70% sequence homology among various mammals. Human TPO contains 332 amino acids (mouse 335) including two internal disulfide bonds and 6 N-linked glycosylation sites (mouse 7). The N-terminal 153 amino acid region of TPO shows structural and amino acid sequence homology to erythropoietin and a pair of arginines (potential cleavage site) separating it from the highly glycosylated carboxyterminal domain.
Thrombopoietin from mouse is an amino acid peptide that plays a pivotal role in megakaryocyte proliferation and differentiation. It also serves as a growth factor for liver endothelial cells in mouse.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin

Nota de análisis

The biological activity was measured in a cell proliferation assay using MO7e cells.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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