Merck
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T5326

Sigma-Aldrich

Anti-γ-Tubulin antibody, Mouse monoclonal

enhanced validation

clone GTU-88, purified from hybridoma cell culture

Synonym(s):
Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

forma del anticuerpo

purified antibody (purified from hybridoma cell culture)

antibody product type

primary antibodies

clon

GTU-88, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 48 kDa

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

envase

antibody small pack of 25 μL

validación mejorada

independent
Learn more about Antibody Enhanced Validation

concentración

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts

isotipo

IgG1

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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Descripción general

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Especificidad

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Inmunógeno

synthetic γ-tubulin peptide, conjugated to KLH

Aplicación

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:
  • Western blot analysis
  • Immunohistochemistry
  • Immunocytology

Acciones bioquímicas o fisiológicas

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

SDS

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