Merck
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T6066

Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Sinónimos:
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Fórmula lineal:
NH2C(CH2OH)3
Número de CAS:
Peso molecular:
121.14
Beilstein:
741883
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

grado

BioPerformance Certified

Nivel de calidad

400

Agency

USP/NF
meets EP testing specifications
meets USP testing specifications

descripción

aminopeptidase substrate

ensayo

≥99.9% (titration)

formulario

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurezas

DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)

pérdida

≤0.5% (loss on drying, 110 °C)

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubilidad

water: soluble (666 mg/ml, yielding a clear colorless solution)

absorción

≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descripción general

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Aplicación

Trizma has been used:
  • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
  • As a component of pre-hybridization buffer in in situ hybridization
  • To suspend dried protein before SDS-PAGE protein characterization
  • As a component of lysis buffer to wash and lyse cells before immunoprecipitation
  • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Envase

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Otras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Recurrent mutations in the MTOR regulator RRAGC in follicular lymphoma.
Peterson LF, et al.
Clinical Cancer Research, 22(21), 5383-5393 (2016)
Culture of human embryonic stem cells on human and mouse feeder cells.
Turksen, Kursad
Human Embryonic Stem Cell Protocols, 91-104 (2006)
Membrane proteins and proteomics: un amour impossible?
Santoni V, et al.
Electrophoresis, 21(6), 1054-1070 (2000)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)

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