Merck
All Photos(3)

T7024

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

Synonym(s):
1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
Linear Formula:
(CH3)2NCH2CH2N(CH3)2
CAS Number:
Molecular Weight:
116.20
Beilstein:
1732991
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.31

grado

for molecular biology

Nivel de calidad

200

línea de producto

BioReagent

ensayo

≥99% (GC)

lim. expl.

9.08 %

índice de refracción

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

densidad

0.775 g/mL at 20 °C (lit.)

actividad extraña

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Aplicación

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Envase

25, 50, 100 mL in glass bottle

Cantidad

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Código de clase de almacenamiento

3 - Flammable liquids

WGK

WGK 1

Punto de inflamabilidad F

61.7 °F - closed cup

Punto de inflamabilidad C

16.5 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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