Merck
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T8660

Sigma-Aldrich

Monoclonal Anti-β-Tubulin III antibody produced in mouse

enhanced validation

clone SDL.3D10, ascites fluid

Synonym(s):
Anti-CDCBM, Anti-CDCBM1, Anti-CFEOM3, Anti-CFEOM3A, Anti-FEOM3, Anti-TUBB4, Anti-beta-4
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

SDL.3D10, monoclonal

contiene

15 mM sodium azide

species reactivity

bovine, rat, human, boar

envase

antibody small pack of 25 μL

validación mejorada

knockout
Learn more about Antibody Enhanced Validation

technique(s)

indirect ELISA: suitable
western blot: 1:400 using a rat brain extract

isotipo

IgG2b

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... TUBB3(10381)
rat ... Tubb3(246118)

Descripción general

Monoclonal Anti-μ Tubulin Isotype III (mouse IgG2b isotype) is derived from the SDL.3D10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.

Especificidad

The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2.

Inmunógeno

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA

Aplicación

It has also been used in immunofluorescence analysis.
Monoclonal Anti-β-Tubulin III antibody produced in mouse has been used in following studies:
  • Immunocytochemistry.
  • Flow cytometry.
  • Immunoblotting.

Acciones bioquímicas o fisiológicas

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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