Merck
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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

peroxidase substrate

Synonym(s):
TMB
NACRES:
NA.83

Nivel de calidad

300

formulario

liquid

condiciones de almacenamiento

protect from light

enviado en

wet ice

temp. de almacenamiento

2-8°C

Aplicación

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Precaución

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Forma física

Ready-to-use.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

Código de clase de almacenamiento

3 - Flammable liquids

WGK

WGK 2

Punto de inflamabilidad F

129.9 °F - closed cup

Punto de inflamabilidad C

54.4 °C - closed cup


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificate of Analysis

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Certificate of Origin

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