51603-U

Supelco

Ascentis® Express Phenyl-Hexyl, 2 μm UHPLC Column

2 μm particle size, L × I.D. 5 cm × 2.1 mm

NACRES:
SB.52

material

stainless steel column

Nivel de calidad

100

Agencia/Método

suitable for USP L11

línea de producto

Ascentis®

característica

endcapped

manufacturer/tradename

Ascentis®

envase

1 ea of

parámetro

1000 bar max. pressure (14500 psi)
60 °C temp. range

aplicaciones

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × D.I.

5 cm × 2.1 mm

impurezas

<5 ppm (metals)

matriz

Fused-Core particle platform
superficially porous particle

grupo activo de la matriz

dimethylphenylhexyl phase

tamaño de partícula

2 μm

tamaño de poro

90 Å pore size

superficie

120 m2/g

intervalo de pH

2 - 9

Industria destacada

Food and Beverages

técnica de separación

reversed phase

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Descripción general

Ascentis® Express 2μm Phenyl-Hexyl UHPLC columns are based on a 90 Å Fused-Core® particle design.The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The bonded, endcapped, dimethylphenylhexyl stationary phase of Ascentis Express 2μm Phenyl-Hexyl provides a stable, reversed phase packing with enhanced p-p and mild steric interactions due to the relatively long-chain hexyl linkage to the base silica. Ascentis Express 2μm Phenyl-Hexyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

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Información legal

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Certificado de Análisis

Certificado de origen

C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I....
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the...
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of...
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of...
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar...
Contenido relacionado
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
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